Mostrando ítems 1-3 de 3

Identification of a novel tailor-made chitinase from white shrimp fenneropenaeus merguiensis

Beygmoradi, Azadeh; Homaei, Ahmad; Hemmati, Roohullah; Del Arco, Jon; Fernández-Lucas, Jesús (Corporación Universidad de la Costa, 2021)

Fenneropenaeus merguiensis (commonly named banana shrimp) is one of the most important farmed crustacean worldwide species for the fisheries and aquaculture industry. Besides its nutritional value, it is a good source of ...
Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean?

Beygmoradi, Azadeh; Homaei, Ahmad; Hemmati, Roohullah; Santos-Moriano, Paloma; Hormigo, Daniel; Fernández-Lucas, Jesús (Universidad de la Costa, 2018-10-01)

Chitinolytic enzymes are capable to catalyze the chitin hydrolysis. Due to their biomedical and biotechnological applications, nowadays chitinolytic enzymes have attracted worldwide attention. Chitinolytic enzymes have ...
¿Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean? (review)

Beygmoradi, Azadeh; Homaei, Ahmad; Hemmati, Roohullah; Santos Moriano, Paloma; Hormigo, Daniel; Fernández Lucas, Jesús (Universidad De La Costa, 2018-09-06)

Chitinolytic enzymes are capable to catalyze the chitin hydrolysis. Due to their biomedical and biotechnological applications, nowadays chitinolytic enzymes have attracted worldwide attention. Chitinolytic enzymes have ...

Envíos recientes

Identification of a novel tailor-made chitinase from white shrimp fenneropenaeus merguiensis
...
Beygmoradi, Azadeh | 2021

Fenneropenaeus merguiensis (commonly named banana shrimp) is one of the most important farmed crustacean worldwide species for the fisheries and aquaculture industry. Besides its nutritional value, it is a good source of chitinase, an enzyme with excellent biological and catalytic properties for many industrial applications. In the present study, a putative chitinase-encoding cDNA was synthesized from mRNA from F. merguiensis hepatopancreas tissue. Subsequently, the corresponding cDNA was cloned, sequenced and functionally expressed in Escherichia coli, and the recombinant F. merguiensis chitinase (rFmCHI) was purified by His-tag affinity chromatography. The bioinformatics analysis of aminoacid sequence of rFmCHI displayed a cannonical multidomain architecture in chitinases which belongs to glycoside hydrolase family 18 (GH18 chitinase). Biochemical characterization revealed rFmCHI as a monomeric enzyme of molecular weight 52 kDa with maximum activity at 40 °C and pH 6.0 Moreover, the recombinant enzyme is also stable up to 60 °C, and in the pH range 5.0-8.0. Steady-state kinetic studies for colloidal chitin revealed KM, Vmax and kcat values of 78.18 μM, 0.07261 μM. min−1 and 43.37 s−1, respectively. Overall, our results aim to demonstrate the potential of rFmCHI as suitable catalyst for bioconversion of chitin waste.

LEER
Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean?
...
Beygmoradi, Azadeh | 2018-10-01

Chitinolytic enzymes are capable to catalyze the chitin hydrolysis. Due to their biomedical and biotechnological applications, nowadays chitinolytic enzymes have attracted worldwide attention. Chitinolytic enzymes have provided numerous useful materials in many different industries, such as food, pharmaceutical, cosmetic, or biomedical industry. Marine enzymes are commonly employed in industry because they display better operational properties than animal, plant, or bacterial homologs. In this mini-review, we want to describe marine chitinolytic enzymes as versatile enzymes in different biotechnological fields. In this regard, interesting comments about their biological role, reaction mechanism, production, functional characterization, immobilization, and biotechnological application are shown in this work.

LEER
¿Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean? (review)
...
Beygmoradi, Azadeh | 2018-09-06

Chitinolytic enzymes are capable to catalyze the chitin hydrolysis. Due to their biomedical and biotechnological applications, nowadays chitinolytic enzymes have attracted worldwide attention. Chitinolytic enzymes have provided numerous useful materials in many different industries, such as food, pharmaceutical, cosmetic, or biomedical industry. Marine enzymes are commonly employed in industry because they display better operational properties than animal, plant, or bacterial homologs. In this mini-review, we want to describe marine chitinolytic enzymes as versatile enzymes in different biotechnological fields. In this regard, interesting comments about their biological role, reaction mechanism, production, functional characterization, immobilization, and biotechnological application are shown in this work.

LEER

Listar por autor "Hemmati, Roohullah"

Identification of a novel tailor-made chitinase from white shrimp fenneropenaeus merguiensis ﻿

Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean? ﻿

¿Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean? (review) ﻿

Envíos recientes

Identification of a novel tailor-made chitinase from white shrimp fenneropenaeus merguiensis

Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean?

¿Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean? (review)

Identification of a novel tailor-made chitinase from white shrimp fenneropenaeus merguiensis

Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean?

¿Marine chitinolytic enzymes, a biotechnological treasure hidden in the ocean? (review)