One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry
Artículo de revista
2018
Catalysts
Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic
compounds. Natural metabolic pathways usually involve complex networks to support cellular
growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of
a wide variety of organic compounds. Methods: The production of different purine nucleosides and
nucleoside-50
-monophosphates has been performed for first time, catalyzed by the sequential action of
2
0
-deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) and hypoxanthine-guanine-xanthine
phosphoribosyltransferase from Thermus themophilus HB8 (TtHGXPRT). Results: The biochemical
characterization of LdNDT reveals that the enzyme is active and stable in a broad range of
pH, temperature, and ionic strength. Substrate specificity studies showed a high promiscuity
in the recognition of purine analogues. Finally, the enzymatic production of different purine
derivatives was performed to evaluate the efficiency of multi-enzymatic system LdNDT/TtHGXPRT.
Conclusions: The production of different therapeutic purine nucleosides was efficiently catalyzed by
LdNDT/TtHGXPRT. In addition, the resulting by-products were converted to IMP and GMP. Taking
all of these features, this bioprocess entails an efficient, sustainable, and economical alternative to
chemical synthetic methods.
- Artículos científicos [3120]
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One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry.pdf
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Título: One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry.pdf
Tamaño: 1.057Mb
PDFLEER EN FLIP