One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry
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Acosta, Javier | 2018
Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic
compounds. Natural metabolic pathways usually involve complex networks to support cellular
growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of
a wide variety of organic compounds. Methods: The production of different purine nucleosides and
nucleoside-50
-monophosphates has been performed for first time, catalyzed by the sequential action of
2
0
-deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) and hypoxanthine-guanine-xanthine
phosphoribosyltransferase from Thermus themophilus HB8 (TtHGXPRT). Results: The biochemical
characterization of LdNDT reveals that the enzyme is active and stable in a broad range of
pH, temperature, and ionic strength. Substrate specificity studies showed a high promiscuity
in the recognition of purine analogues. Finally, the enzymatic production of different purine
derivatives was performed to evaluate the efficiency of multi-enzymatic system LdNDT/TtHGXPRT.
Conclusions: The production of different therapeutic purine nucleosides was efficiently catalyzed by
LdNDT/TtHGXPRT. In addition, the resulting by-products were converted to IMP and GMP. Taking
all of these features, this bioprocess entails an efficient, sustainable, and economical alternative to
chemical synthetic methods.
LEER