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dc.creatordel Arco, Jon
dc.creatorGalindo, Javier
dc.creatorClemente Suárez, Vicente Javier
dc.creatorCorrales, Amaira
dc.creatorFernández-Lucas, Jesús
dc.date.accessioned2019-08-09T15:38:21Z
dc.date.available2019-08-09T15:38:21Z
dc.date.issued2019-07-09
dc.identifier.urihttp://hdl.handle.net/11323/5139
dc.description.abstractNowadays enzymatic synthesis of nucleic acid derivatives is gaining momentum over traditional chemical synthetic processes. Biotransformations catalyzed by whole cells or enzymes offer an ecofriendly and efficient alternative to the traditional multistep chemical methods, avoiding the use of chemical reagents and organic solvents that are expensive and environmentally harmful. Herein we report for the first time the covalent immobilization a uracil phosphoribosyltransferase (UPRT). In this sense, UPRT from Thermus thermophilus HB8 was immobilized onto glutaraldehyde-activated MagReSyn®Amine magnetic iron oxide porous microparticles (MTtUPRT). According to the catalyst load experiments, MTtUPRT3 was selected as optimal biocatalyst for further studies. MTtUPRT3 was active and stable in a broad range of temperature (70–100 °C) and in the pH interval 6–8, displaying maximum activity at 100 °C and pH 7 (activity 968 IU/gsupport, retained activity 100%). In addition, MTtUPRT3 could be reused up to 8 times in the synthesis of uridine-5′-monophosphate (UMP). Finally, MTtUPRT3 was successfully applied in the sustainable synthesis of different 5-modified uridine-5′-monophosphates at short times. Taking into account these results, MTtUPRT3 would emerge as a valuable biocatalyst for the synthesis of nucleoside monophosphates through an efficient and environmentally friendly methodology.es_ES
dc.language.isoenges_ES
dc.publisherUniversidad de la Costaes_ES
dc.relation.ispartofhttps://doi.org/10.1016/j.bbapap.2019.07.004es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectBiocatalysises_ES
dc.subjectEnzyme immobilizationes_ES
dc.subjectPhosphoribosyltransferasees_ES
dc.subjectNucleic acid derivativeses_ES
dc.subjectActive pharmaceutical ingredientses_ES
dc.titleSustainable synthesis of uridine-5′-monophosphate analogues by immobilized uracil phosphoribosyltransferase from Thermus thermophiluses_ES
dc.typePreprintes_ES
dc.type.hasVersioninfo:eu-repo/semantics/draftes_ES


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