Sustainable production of nucleoside analogues by a high-efficient purine 2′- deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticles
Pre-Publicación
2019-10
Universidad de la Costa
The present work aims to develop a magnetic biocatalyst for customized production of nucleoside analogues
using mutant His-tagged purine 2′-deoxyribosyltransferase from Trypanosoma brucei (TbPDTV11S) immobilized
onto Ni2+ chelate magnetic iron oxide porous microparticles (MTbPDTV11S). Biochemical characterization revealed MTbPDTV11S5 as optimal candidate for further studies (10,552 IU g−1; retained activity 54% at 50 °C and
pH 6.5). Interestingly, MTbPDTV11S5 displayed the highest activity value described up to date for an immobilized
NDT. Moreover, MTbPDTV11S5 was successfully employed in the one-pot, one-step production of different
therapeutic nucleoside analogues, such as cladribine or 2′-deoxy-2-fluoroadenosine, among others. Finally,
MTbPDTV11S5 proved to be stable when stored at 50 °C for 8 h and pH 6.0 and reusable up to 10 times without
negligible loss of activity in the enzymatic production of the antitumor prodrug 2′-deoxy-2-fluoroadenosine
- Artículos científicos [3120]
Descripción:
Sustainable production of nucleoside analogues by a high-efficient purine 2′- deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticles.pdf
Título: Sustainable production of nucleoside analogues by a high-efficient purine 2′- deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticles.pdf
Tamaño: 181.5Kb
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Título: Sustainable production of nucleoside analogues by a high-efficient purine 2′- deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticles.pdf
Tamaño: 181.5Kb
PDFLEER EN FLIP
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