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Sustainable production of nucleoside analogues by a high-efficient purine 2′- deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticles

dc.contributor.authorDel Arco, Jonspa
dc.contributor.authorJordaan, Justinspa
dc.contributor.authorMoral-Dardé, Verónicaspa
dc.contributor.authorFernández-Lucas, Jesússpa
dc.date.accessioned2019-08-09T15:55:20Z
dc.date.available2019-08-09T15:55:20Z
dc.date.issued2019-10
dc.identifier.urihttp://hdl.handle.net/11323/5140spa
dc.description.abstractThe present work aims to develop a magnetic biocatalyst for customized production of nucleoside analogues using mutant His-tagged purine 2′-deoxyribosyltransferase from Trypanosoma brucei (TbPDTV11S) immobilized onto Ni2+ chelate magnetic iron oxide porous microparticles (MTbPDTV11S). Biochemical characterization revealed MTbPDTV11S5 as optimal candidate for further studies (10,552 IU g−1; retained activity 54% at 50 °C and pH 6.5). Interestingly, MTbPDTV11S5 displayed the highest activity value described up to date for an immobilized NDT. Moreover, MTbPDTV11S5 was successfully employed in the one-pot, one-step production of different therapeutic nucleoside analogues, such as cladribine or 2′-deoxy-2-fluoroadenosine, among others. Finally, MTbPDTV11S5 proved to be stable when stored at 50 °C for 8 h and pH 6.0 and reusable up to 10 times without negligible loss of activity in the enzymatic production of the antitumor prodrug 2′-deoxy-2-fluoroadenosinespa
dc.language.isoeng
dc.publisherUniversidad de la Costaspa
dc.relation.ispartofhttps://doi.org/10.1016/j.biortech.2019.121772spa
dc.rightsCC0 1.0 Universalspa
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/spa
dc.subjectEnzyme immobilizationspa
dc.subjectBioprocessesspa
dc.subjectNucleoside analoguesspa
dc.subject2′-Deoxyribosyltransferasesspa
dc.titleSustainable production of nucleoside analogues by a high-efficient purine 2′- deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticlesspa
dc.typePre-Publicaciónspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.identifier.instnameCorporación Universidad de la Costaspa
dc.identifier.reponameREDICUC - Repositorio CUCspa
dc.identifier.repourlhttps://repositorio.cuc.edu.co/spa
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Modifying enzyme activity and selectivity by immobilization. Chem. Soc. Rev. 42 (15), 6290–6307. Santos, J.C.S., Barbosa, O., Ortiz, C., Berenguer-Murcia, A., Rodrigues, R.C., FernandezLafuente, R., 2015. Importance of the support properties for immobilization or purification of enzymes. Chem. Cat. Chem. 7 (16), 2413–2432. Stepankova, V., Bidmanova, S., Koudelakova, T., Prokop, Z., Chaloupkova, R., Damborsky, J., 2013. Strategies for stabilization of enzymes in organic solvents. ACS Catal. 3 (12), 2823–2836.spa
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dc.type.redcolhttp://purl.org/redcol/resource_type/ARTOTRspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa


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