Listar por autor "Acosta, Javier"
Mostrando ítems 1-7 de 7
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Enzymatic Production Of Non-Natural Nucleoside-5′-Monophosphates By A Thermostable Uracil Phosphoribosyltransferase
Del Arco, Jon; Acosta, Javier; D'Muniz Pereira, Humberto; Perona, Almudena; Lokanath, Neratur Krishnappagowda; Kunishima, Naoki; Fernandez Lucas, Jesus (ChemCatChem, 2018-06-23)The use of enzymes as biocatalysts applied to synthesis of modified nucleoside-5′-monophosphates (NMPs) is an interesting alternative to traditional multistep chemical methods which offers several advantages, such as ... -
Hypoxanthine-Guanine Phosphoribosyltransferase/adenylate Kinase From Zobellia galactanivorans: A Bifunctional Catalyst for the Synthesis of Nucleoside-5′-Mono-, Di- and Triphosphates
Acosta, Javier; Del Arco, Jon; Del Pozo, Maria Luisa; Herrera, Beliña; Clemente-Suárez, Vicente Javier; Berenguer, José; Hidalgo, Aurelio; Fernández-Lucas, Jesús (Frontiers in Bioengineering and Biotechnology, 2020-06-24)In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from ... -
Molecular basis of Ndt-mediated activation of nucleoside-based prodrugs and application in suicide gene therapy
Acosta, Javier; Pérez, Elena; Sánchez-Murcia, Pedro Alejandro; Fillat, Cristina; Fernández-Lucas, Jesús (Corporación Universidad de la Costa, 2021)Herein we report the first proof for the application of type II 2′-deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) in suicide gene therapy for cancer treatment. To this end, we first confirm the hydrolytic ... -
N-Ribosyltransferase From Archaeoglobus veneficus: A Novel Halotolerant and Thermostable Biocatalyst for the Synthesis of Purine Ribonucleoside Analogs
Acosta, Javier; Del Arco, Jon; Pisabarro, Victor; Gago, Federico; Fernández-Lucas, Jesús (Frontiers in Bioengineering and Biotechnology, 2020-06-20)Nucleoside-2′-deoxyribosyl-transferases (NDTs) catalyze a transglycosylation reaction consisting of the exchange of the 2′-deoxyribose moiety between a purine and/or pyrimidine nucleoside and a purine and/or pyrimidine ... -
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases
Del Arco, Jon; Acosta, Javier; Fernández-Lucas, Jesús (Corporación Universidad de la Costa, 2021-01-27)Nowadays, pharmaceutical industry demands competitive and eco-friendly processes for active pharmaceutical ingredients (APIs) manufacturing. In this context, enzyme and whole-cell mediated processes offer an efficient, ... -
One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry
Acosta, Javier; Del Arco, Jon; Martínez Pascual, Sara; Clemente Suárez, Vicente Javier; Fernandez Lucas, Jesus (Catalysts, 2018)Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic compounds. Natural metabolic pathways usually involve complex networks to support cellular growth and survival. In this regard, ... -
Taylor-made production of pyrimidine nucleoside-5′-monophosphate analogues by highly stabilized mutant uracil phosphoribosyltransferase from Toxoplasma gondii
Acosta, Javier; Nguyen, Kim; C. Spitale, Robert; Fernández-Lucas, Jesús (Bioresource Technology, 2021)Nowadays, enzymatic synthesis of nucleotides is an efficient and sustainable alternative to chemical methodologies. In this regard, after the biochemical characterization of wild-type and mutant uracil phosphoribosyltransferases ...