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dc.creatorDel Arco, Jon
dc.creatorSánchez Murcia, Pedro Alejandro
dc.creatorMancheño José Miguel, José Miguel
dc.creatorGago, Federico
dc.creatorFernandez Lucas, Jesus
dc.date.accessioned2018-11-16T01:03:25Z
dc.date.available2018-11-16T01:03:25Z
dc.date.issued2018-05-23
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/11323/1093
dc.description.abstractIn our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2′-deoxynucleosides but is also proficient using cytosine and 2′-deoxycytidine as substrates. These unusual features preclude the strict classification of CtNDT as either a type I or a type II NDT and further suggest that this simple subdivision may need to be updated in the future. Our findings also hint at a possible link between oligomeric state and NDT’s substrate specificity. Interestingly from a practical perspective, CtNDT displays high activity (80–100%) in the presence of several water-miscible co-solvents in a proportion of up to 20% and was successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine.es_CO
dc.language.isoengen_US
dc.publisherApplied Microbiology And Biotechnologyen_US
dc.rightsAtribución – No comercial – Compartir igualen_US
dc.subjectEnzymatic Synthesisen_US
dc.subjectExtremophilesen_US
dc.subjectHomology Modelingen_US
dc.subjectNucleoside 2′-Deoxyribosyltransferaseen_US
dc.subjectNucleoside Analoguesen_US
dc.titleCharacterization Of An Atypical, Thermostable, Organic Solvent- And Acid-Tolerant 2′-Deoxyribosyltransferase From Chroococcidiopsis Thermalisen_US
dc.typearticleen_US


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