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Selective Fractionation And Isolation Of Allelopathic Compounds From Helianthus Annuus L. Leaves By Means Of High-Pressure Techniques


Fuentes Gándara, Fabio Armando
Torres, Ascension
Fernández Ponce, M. T.
Casas, L.
Mantell Serrano, Casimiro
Varela, Rosa Maria
Martinez De La Ossa Fernandez, E. J
Macias, Francisco A.

Artículo de revista

2019

Journal Of Supercritical Fluids

https://doi.org/https://doi.org/10.1016/j.supflu.2018.08.004

08968446

AllelopathyBuscar en Repositorio UMECIT
Enhanced Solvent ExtractionBuscar en Repositorio UMECIT
FractionationBuscar en Repositorio UMECIT
Helianthus AnnusBuscar en Repositorio UMECIT
Supercritical FluidsBuscar en Repositorio UMECIT

The allelopathic potential of Helianthus annuus L. leaves was study based on bio-directed chemical fractionation approach. Aerial parts of H. annuus were extracted by means of SFE using supercritical carbon dioxide (scCO2) and ESE using CO2+50% EtOH/H2O (varying ethanol in water from 0 to 100%). Extractions were carried out at 400 bar, 55 °C, 20 g/min and for 4 h. Then, extracts were fractionated in three separators at the following conditions: S1: 200 bar/45 °C; S2: 90 bar/40 °C; and S3: 1 atm/30 °C. ESE obtained higher overall yields than scCO2 and the use of water as cosolvent (CO2+50% H2O) resulted in a S3 fraction free from chlorophylls and rich in bioactive compounds. 14 compounds, including fatty acids, terpenes, flavonoids and heliannuols, were isolated from this fraction. After performing the bioassay on pure compounds, heliannuol D, tambulin, pinoresinol and sesquiterpene 10-oxo-isodauc-3-en-15-al showed the most effective inhibitor profiles.

http://hdl.handle.net/11323/1275

  • Artículos científicos [2641]

Descripción: Selective Fractionation And Isolation.pdf
Título: Selective Fractionation And Isolation.pdf
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