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dc.creatorVisbal Acevedo, Renso Raul
dc.creatorFernández Moreira, Vanesa
dc.creatorMarzo, Isabel
dc.creatorLaguna, Antonio
dc.creatorGimeno, María Concepción
dc.date.accessioned2018-11-20T15:05:59Z
dc.date.available2018-11-20T15:05:59Z
dc.date.issued2016
dc.identifier.issn14779226
dc.identifier.urihttp://hdl.handle.net/11323/1417
dc.description.abstractA range of fluorescent and biologically compatible gold(i)-N-heterocyclic carbenes bearing acridine as a wingtip group and either a 2-mercaptopyridine or a tetra-O-acetyl-1-thio-β-d-glucopyranoside as an ancillary ligand has been synthesised. Their luminescence, cytotoxicity and biodistribution have been investigated together with those of analogous gold(i) and silver(i) chloride- and bis-NHC complexes. All complexes displayed emissions based on IL transitions centred on the acridine moiety. The cytotoxic activity measured in lung, A549, and pancreatic, MiaPaca2, carcinoma cell lines revealed a general cytotoxicity pattern (thiolate > biscarbene > chloride derivatives) and flow cytometry assays pointed towards apoptosis as the cell death mechanism. Moreover, fluorescence cell microscopy disclosed an unusual biodistribution behavior, being mainly localised in lysosomes and to a lesser extent in the nucleus. Preliminary DNA interaction experiments suggested the metal fragment and not the acridine moiety as responsible for such biodistribution, which widen the scope for new biological targets.spa
dc.language.isoengeng
dc.publisherDalton Transactionseng
dc.rightsAtribución – No comercial – Compartir igualeng
dc.subjectCell cultureeng
dc.subjectCell deatheng
dc.subjectChlorine compoundseng
dc.subjectFluorescenceeng
dc.subjectGoldeng
dc.subjectGold compoundseng
dc.subjectLigandseng
dc.subjectLuminescenceeng
dc.subjectOrganic compoundseng
dc.subjectSilvereng
dc.titleCytotoxicity and biodistribution studies of luminescent Au(i) and Ag(i) N-heterocyclic carbenes. Searching for new biological targetseng
dc.typeArticleeng
dc.identifier.doihttps://doi.org/10.1039/C6DT02878K


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