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dc.creatorT. Toloza, Carlos A.
dc.creatorS. Almeida, Joseany M.
dc.creatorP. Silva, Lillian O.
dc.creatorMacedo, Rosana C.
dc.creatorLamounier, Ana Paula
dc.creatorAucelio, Ricardo Q.
dc.creatorda Cunha, Alessandra L. M. C.
dc.date.accessioned2020-03-31T15:33:52Z
dc.date.available2020-03-31T15:33:52Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/11323/6145
dc.description.abstractA high-performance chromatographic method was developed to determine the fungicide kresoxim-methyl. Off-line photochemical derivatization was used to induce the formation of a stable and fluorescent product since the fungicide does not present natural fluorescence. Intense fluorescence at 370/430nm was achieved by treating the analyte in solution at pH 6 to ultraviolet light for 45s. The chromatographic conditions included isocratic elution with 50/ 50% (v/v) acetonitrile/water and the photochemical product appeared at a retention time of 7.2min. The short and long term stabilities of the photoproduct were evaluated and variation of less than 5% was achieved. The limits of detection in water samples and in grapes samples were 0.019mg kg1 and 0.065mg kg1 of kresoxim-methyl residue, respectively. The linear response covered three orders of magnitude up to 10.6mg kg1 of kresoxim-methyl. The robustness was evaluated through a Box–Behnken experimental design showing the insignificance of all factors and their interactions. The potential interference of tebuconazole for the determination of kresoxim-methyl was studied. The use of the dispersive liquid-liquid microextraction (DLLME) allowed recoveries between 80% and 101% depending on concentration with the minimum generation of waste products.spa
dc.language.isoengspa
dc.publisherUniversidad de la Costaspa
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectDispersive liquid-liquid microextraction (DLLME)spa
dc.subjectHigh-performance liquid chromatography (HPLC)spa
dc.subjectKresoxim-methylspa
dc.subjectPhotochemical-induced fluorescencespa
dc.subjectStrobirulinspa
dc.titleDetermination of Kresoxim-Methyl in Water and in Grapes by High-Performance Liquid Chromatography (HPLC) Using PhotochemicalInduced Fluorescence and Dispersive Liquid-Liquid Microextraction (DLLME)spa
dc.typePreprintspa
dc.type.hasVersioninfo:eu-repo/semantics/draftspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa


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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal