Show simple item record

dc.creatorT. Toloza, Carlos A.
dc.creatorS. Almeida, Joseany M.
dc.creatorP. Silva, Lillian O.
dc.creatorMacedo, Rosana C.
dc.creatorLamounier, Ana Paula
dc.creatorAucelio, Ricardo Q.
dc.creatorda Cunha, Alessandra L. M. C.
dc.description.abstractA high-performance chromatographic method was developed to determine the fungicide kresoxim-methyl. Off-line photochemical derivatization was used to induce the formation of a stable and fluorescent product since the fungicide does not present natural fluorescence. Intense fluorescence at 370/430nm was achieved by treating the analyte in solution at pH 6 to ultraviolet light for 45s. The chromatographic conditions included isocratic elution with 50/ 50% (v/v) acetonitrile/water and the photochemical product appeared at a retention time of 7.2min. The short and long term stabilities of the photoproduct were evaluated and variation of less than 5% was achieved. The limits of detection in water samples and in grapes samples were 0.019mg kg1 and 0.065mg kg1 of kresoxim-methyl residue, respectively. The linear response covered three orders of magnitude up to 10.6mg kg1 of kresoxim-methyl. The robustness was evaluated through a Box–Behnken experimental design showing the insignificance of all factors and their interactions. The potential interference of tebuconazole for the determination of kresoxim-methyl was studied. The use of the dispersive liquid-liquid microextraction (DLLME) allowed recoveries between 80% and 101% depending on concentration with the minimum generation of waste
dc.publisherUniversidad de la Costaspa
dc.rightsCC0 1.0 Universal*
dc.subjectDispersive liquid-liquid microextraction (DLLME)spa
dc.subjectHigh-performance liquid chromatography (HPLC)spa
dc.subjectPhotochemical-induced fluorescencespa
dc.titleDetermination of Kresoxim-Methyl in Water and in Grapes by High-Performance Liquid Chromatography (HPLC) Using PhotochemicalInduced Fluorescence and Dispersive Liquid-Liquid Microextraction (DLLME)spa

Files in this item


This item appears in the following Collection(s)

Show simple item record

CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal