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dc.creatorFernandes Caldeira, Dayene de Assis
dc.creatorMuniz Mesquita, Flávia
dc.creatorGomes Pinheiro, Felipe
dc.creatorFerreira Oliveira, Dahienne
dc.creatorSilva Oliveira, Luis Felipe
dc.creatorMatheus Nascimento, Jose Hamilton
dc.creatorMaeda Takiya, Christina
dc.creatorMaciel, Leonardo
dc.creatorAraujo Zin, Walter
dc.date.accessioned2020-12-29T20:09:27Z
dc.date.available2020-12-29T20:09:27Z
dc.date.issued2020-11-28
dc.identifier.urihttps://hdl.handle.net/11323/7648
dc.description.abstractC60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and in vivo. We hypothesized that C60 impairs lung mechanics and mitochondrial function. Thirty-five male BALB/c mice were randomly divided into two groups intratracheally instilled with vehicle (0.9% NaCl + 1% Tween 80, CTRL) or C60 (1.0 mg/kg, FUL). Twenty-four hours after exposure, 15 FUL and 8 CTRL mice were anesthetized, paralyzed, and mechanically ventilated for the determination of lung mechanics. After euthanasia, the lungs were removed en bloc at end-expiration for histological processing. Lung tissue elastance and viscance were augmented in FUL group. Increased inflammatory cell number, alveolar collapse, septal thickening, and pulmonary edema were detected. In other six FUL and six CTRL mice, mitochondria expressed reduction in state 1 respiration [FUL = 3.0 ± 1.14 vs. CTRL = 4.46 ± 0.9 (SEM) nmol O2/min/mg protein, p = 0.0210], ATP production (FUL = 122.6 ± 18 vs. CTRL = 154.5 ± 14 μmol/100 μg protein, p = 0.0340), and higher oxygen consumption in state 4 [FUL = 12.56 ± 0.9 vs. CTRL = 8.26 ± 0.6], generation of reactive oxygen species (FUL 733.1 ± 169.32 vs. CTRL = 486.39 ± 73.1 nmol/100 μg protein, p = 0.0313) and reason ROS/ATP [FUL = 8.73 ± 2.3 vs. CTRL = 2.99 ± 0.3]. In conclusion, exposure to fullerene C60 impaired pulmonary mechanics and mitochondrial function, increased ROS concentration, and decrease ATP production.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherCorporación Universidad de la Costaspa
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.sourceNanotoxicologyspa
dc.subjectFullerene C60spa
dc.subjectLung mechanicsspa
dc.subjectAlveolar collapsespa
dc.subjectMitochondrial functionspa
dc.subjectATP productionspa
dc.subjectReactive oxygen speciesspa
dc.titleAcute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanicsspa
dc.typePreprintspa
dc.type.hasVersioninfo:eu-repo/semantics/draftspa
dc.source.urlhttps://www.tandfonline.com/doi/full/10.1080/17435390.2020.1863498spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.identifier.doihttps://doi.org/10.1080/17435390.2020.1863498


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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal