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dc.creatorNguyen, Kim
dc.creatorKubota, Miles
dc.creatorDel Arco, Jon
dc.creatorFeng, Chao
dc.creatorSingha, Monika
dc.creatorBeasley, Samantha
dc.creatorSakr, Jasmine
dc.creatorP. Gandhi, Sunil
dc.creatorBlurton-Jones, Mathew
dc.creatorFernández Lucas, Jesus
dc.creatorC. Spitale, Robert
dc.date.accessioned2021-02-19T16:50:53Z
dc.date.available2021-02-19T16:50:53Z
dc.date.issued2020-11-21
dc.identifier.urihttps://hdl.handle.net/11323/7879
dc.description.abstractProfiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherCorporación Universidad de la Costaspa
dc.relation.ispartofhttps://pubs.acs.org/toc/acbcct/15/12spa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceACS Chemical Biologyspa
dc.subjectPeptides and proteinsspa
dc.subjectGeneticsspa
dc.subjectLabelingspa
dc.subjectUracilspa
dc.subjectImaging probesspa
dc.titleA bump-hole strategy for increased stringency of cell-specific metabolic labeling of rnaspa
dc.typearticlespa
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dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.source.urlhttps://pubs.acs.org/doi/10.1021/acschembio.0c00755#spa
dc.rights.accessrightsinfo:eu-repo/semantics/embargoedAccessspa
dc.identifier.doihttps://doi.org/10.1021/acschembio.0c00755
dc.date.embargoEnd2021-11-21


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